USE OF IMAGEJ, SMARTPHONE CAMERA, AND LIGHT MICROSCOPE TO EVALUATE STALLION SPERM CONCENTRATION
نویسندگان
چکیده
منابع مشابه
Measurement of Sperm Concentration in Stallion Ejaculates Using Photometric or Direct Sperm Enumeration Techniques
A highly significant correlation was detected among photometric and direct sperm enumeration techniques for evaluating sperm concentration. Depending on the instrument used, the photometric techniques tended to overestimate or underestimate sperm concentration when compared to a hemacytometer standard. Precautions should be taken when using techniques other than a hemacytometer to measure sperm...
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After a serious injury or sudden death, epididymis cauda sperm recovery and cryopreservation may present as the last opportunity to obtain genetic material from a valuable stallion. This study evaluated the viability of cooled equine sperm collected by three different methods: sperm of ejaculated (G1), sperm recovered from the epididymal cauda immediately after orchiectomy (G2) and sperm recove...
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Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytom...
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companion volumes could be prepared for other countries. Some of the sources available for France may not exist elsewhere, but it would be worth exploring the possibilities. For example, an analysis is included of 85 letters written by patients to a doctor over the period 1780 to 1830 (p. 19). An indication is given of the tone of the letters, of which parts of the body they mention, and of the...
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OBJECTIVE The presence of sperm-head vacuoles has been suspected to be deleterious to the outcomes of assisted reproductive technology (ART). It is difficult to accurately distinguish morphologically abnormal sperm with vacuoles under a light microscope. This study was performed to analyze the result of the observation of sperm-head vacuoles using Papanicolaou staining under a light microscope ...
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ژورنال
عنوان ژورنال: Archives of Veterinary Science
سال: 2020
ISSN: 2317-6822,1517-784X
DOI: 10.5380/avs.v25i3.71839